Bioanalytical chemistry

Bioanalytical chemistry: Eavesdropping on interactions.

Applications of Lasers in Bioanalytical Chemistry

ground due to oxygen. This background ECL however, does not prevent one from obtaining peroxide detection limits below I ,aM. The ECL intensity varies with both pH and potential. The net ECL to background ECL ratio is a maximum at pH 10. Gold and glassy carbon electrodes are preferable to platinum, but gold is somewhat better than glassy carbon. Potentials more negative than +0.3 V yield insign...

Bioanalytical chemistry: Protein oligomers frozen in time.

When Fenn and co-workers recorded early electrospray ionization (ESI) mass spectra of biopolymers, they observed what are now characteristic charge-state distributions as well as the formation of non-covalent complexes that persisted into the gas phase1. Writing on page 326 of this issue2, Bernstein et al. describe the remarkable diff erences in the distributions of oligomers formed by diff ere...

Published in Analytical and Bioanalytical Chemistry May 2013, Volume 405, Issue 13, pp 4627-4638

1 Characterization of grape seed procyanidins by comprehensive two-dimensional hydrophilic interaction x reversed phase liquid chromatography coupled to diode array detection and tandem mass spectrometry (HILICxRPLC-DAD-MS/MS). Lidia Montero, Miguel Herrero*, Marin Prodanov, Elena Ibáñez, Alejandro Cifuentes Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC-UAM), Nicolás C...

Bioanalytical method validation

A syntetic discussion on bioanalytical methods validation is presented from the point of view of regulatory documents, scientific articles and books. The validation parameters are described, together with an example of validation methodology applied in the case of chromatographic methods used in bioanalysis, taking in account to the recent Food and Drug Administration (FDA) guidelines and docum...